Protein Details: Glutamate receptor ionotropic; NMDA 2A

Protein ID

ICDB_Pro_0730

Protein Name

Glutamate receptor ionotropic; NMDA 2A

Gene Name

Grin2a

Organism

Rattus norvegicus (Rat)

Length

1464 amino acids

AlphaFoldDB

AF-Q00959-F1-model_v4.pdb

Function

Component of NMDA receptor complexes that function as heterotetrameric; ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit; glycine binding to the zeta subunit; plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have lower sensitivity to glutamate and faster deactivation kinetics than channels formed by GRIN1 and GRIN2B. Contributes to the slow phase of excitatory postsynaptic current; long-term synaptic potentiation; and learning (By similarity). Participates in the synaptic plasticity regulation through activation by the L-glutamate releaseed by BEST1; into the synaptic cleft; upon F2R/PAR-1 activation in astrocyte (By similarity).

Sequence

MGRLGYWTLLVLPALLVWRDPAQNAAAEKGPPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQAEKPETPLHTLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPYMHQYMTRFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFASTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNMNSSRMDSPKRATDFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGDNMNELQTFVANRHKDNLSNYVFQGQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNPVSQRDEKTAENRTHSLKSPRYLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKRSMASKYPKDCSDVDRTYMKTKASSPRDKIYTIDGEKEPSFHLDPPQFVENITLPENVGFPDTYQDHNENFRKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKHFTLKDKGSPHSEGSDRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPATREEVYQQDWSQNNALQFQKNKLRINRQHSYDNILDKPREIDLSRPSRSISLKDRERLLEGNLYGSLFSVPSSKLLGNKSSLFPQGLEDSKRSKSLLPDHASDNPFLHTYGDDQRLVIGRCPSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKNTMYSTPRVLNSCSNRRVYKKMPSIESDV

PDB Structures

Ligand Binding

1. DICL_CP

2. DICL_Pep

Binding Site

BINDING 44; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; BINDING 128; /ligand="Zn(2+)"; BINDING 511..513; /ligand="L-glutamate"; BINDING 689..690; /ligand="L-glutamate"

Disease

Location

Detected in brain cortex; olfactory bulb; hippocampus including the dentate gyrus; striatum; thalamus; superior colliculus; inferior colliculus; midbrain and cerebellum (at protein level) (PubMed:22960932; PubMed:9509416). Detected in brain cortex; hypothalamus and cerebellum (PubMed:1350383).

DOI ID

10.1126/science.256.5060.1217; 10.1016/s0021-9258(18)53849-7; 10.1126/science.7569905; 10.1074/jbc.273.33.21105; 10.1006/mcne.1998.0679; 10.1523/jneurosci.21-04-01228.2001; 10.1523/jneurosci.21-20-07985.2001; 10.1124/mol.62.5.1119; 10.1152/jn.00531.2001; 10.1523/jneurosci.23-31-10064.2003; 10.1523/jneurosci.3799-05.2006; 10.1038/ncomms1871; 10.1038/ncomms10181; 10.1038/nn.3214; 10.1016/j.neuron.2017.03.018; 10.1016/j.str.2019.04.001; 10.1038/nature04089; 10.1124/mol.113.085803; 10.1016/j.neuron.2013.11.033; 10.1016/j.neuron.2016.08.014; 10.1016/j.neuron.2016.11.006; 10.1124/mol.116.107912; 10.1073/pnas.1707752114

RefSeq

NP_036705.3